Research group ThieleLab

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  1. J. Thiele*: „The challenge of cytocompatibility in high-resolution additive manufacturing“, in preparation.
  2. A. Kaufmann, C. Graf, S. Manoilenko, M. Milic, L. Schäfer, M. Männel, N. Hauck, T. Heida, D. Hahn, M. Dippe, J. Thiele*: „Microbioreactors for polyketide synthesis based on enzyme-functionalized microgel cascades“, in preparation.
  3. N. Hauck, G. Cojoc, T. Beck, R. Schlüßler, S. Ahmed, S. Abuhattum, I. Raguzin, M. Mayer, J. Schubert, P. Müller, J. Guck*, J. Thiele*: „PNIPAAm microgels with defined network architecture for temperature sensing in optical stretchers“, in preparation.
  4. T. A. Neuendorf, N. Weigel, M. Vigogne, J. Thiele*: „Additive soft matter design by UV-induced polymer hydrogel inter-crosslinking“, submitted.
  5. D. Rettke, C. Danneberg, T. A. Neuendorf, S. Kühn, J. Friedrichs, N. Hauck, C. Werner, J. Thiele, T. Pompe*: „Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors based on reflection interference contrast microscopy“, revisions.


  1. E. Stengelin, J. Thiele, S. Seiffert*: „Multiparametric material functionality of microtissue-based in-vitro models as alternatives to animal testing“, Adv. Sci. 2022, 2105319. DOI: 10.1002/advs.202105319.


  1. N. Hauck, T. A. Neuendorf, M. J. Männel, L. Vogel, P. Liu, E. Stündel, Y. Zhang, J. Thiele*: „Processing of fast-gelling hydrogel precursors in microfluidics by electrocoalescence of reactive species“, Soft Matter 2021, 17, 10312. DOI: 10.1039/d1sm01176f.
  2. M. J. Männel, N. Weigel, N. Hauck, T. Heida, J. Thiele*: „Combining Hydrophilic and Hydrophobic Materials in 3D printing for Fabricating Microfluidic Devices with Spatial Wettability“, Adv. Mater. Technol. 2021, 6(9), 2100094. DOI: 10.1002/admt.202100094.
  3. N. Weigel, M. J. Männel, J. Thiele*: „Flexible materials for high-resolution 3D printing of microfluidic devices with integrated droplet size regulation“, ACS Appl. Mater. Interfaces 2021, 13, 31086. DOI: 10.1021/acsami.1c05547.
  4. M. J. Männel, E. Baysak, J. Thiele*: „Fabrication of microfluidic devices for emulsion formation by micro-stereolithography“, Molecules 2021, 26(9), 2817. DOI: 10.3390/molecules26092817.
  5. Y. Li*, M. J. Männel, N. Hauck, H. P. Patel, G. K. Auernhammer, S. Chae, A. Fery, J. Li*, J. Thiele*: „Embedment of quantum dots and biomolecules in an in-situ formed dipeptide hydrogel using microfluidics“, Angew. Chem. Int. Ed. 2021, 60(12), 6724. DOI: 10.1002/ange.202015340.
  6. D. Eigel, R. Schuster, M. J. Männel, J. Thiele, M. Panasiuk, L. Andrae, C. Varracchio, A. Brancale, P. B. Welzel, W. Huttner, C. Werner, B. Newland*, K. R. Long*: „Sulfonated cryogel scaffolds for focal delivery in ex-vivo brain tissue cultures“, Biomaterials 2021, 271, 120712. DOI: 10.1016/j.biomaterials.2021.120712.
  7. N. Weigel, J. Thiele*: „Photopolymer formulations for μSL printing of hydrogel microstructures as swellable functional elements“, Proc. SPIE 11637, Microfluidics, BioMEMS, and Medical Microsystems XIX 2021, 116370A. DOI: 10.1117/12.2576485.


  1. T. Köhler, T. Heida, S. Hoefgen, N. Weigel, V. Valiante, J. Thiele*: „Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion“, RSC Adv. 2020, 10, 40588. DOI: 10.1039/d0ra06702d.
  2. C. Huster, D. Rekhade, A. Hausch, S. Ahmed, N. Hauck, J. Thiele, J. Guck, K. Kroy, G. Cojoc*: „Stretching and Heating Cells with Light Nonlinear Photothermal Cell Rheology“, New J. Phys. 2020, 22, 085003. DOI: 10.1088/1367-2630/aba14b.
  3. T. Heida, O. Otto, D. Biedenweg, N. Hauck, J. Thiele*: „Microfluidic fabrication of click chemistry-mediated hyaluronic acid microgels: A bottom-up material guide to tailor a microgel’s physicochemical and mechanical properties“, Polymers 2020, 12(8), 1760. DOI: 10.3390/polym12081760.
  4. J. E. Kufs, S. Hoefgen, J. Rautschek, A. U. Bissell, C. Graf, J. Fiedler, D. Braga, L. Regestein, M. A. Rosenbaum, J. Thiele, V. Valiante*: „Rational design of flavonoid production routes using combinatorial and precursor-directed biosynthesis“, ACS Synth. Biol. 2020, 9, 1823. DOI: 10.1021/acssynbio.0c00172.
  5. T. Köhler, P. A. Patsis, D. Hahn, A. Ruland, M. Müller, J. Thiele*: „DNAzymes as catalysts for L-tyrosine and amyloid beta oxidation“, ACS Omega 2020, 5(13), 7059. DOI: 10.1021/acsomega.9b02645.
  6. M. J. Männel, C. Fischer, J. Thiele*: „A Non-Toxic Resin for micro-stereolithography for Cell Cultures of HUVECs“, Micromachines 2020, 11(3), 246. DOI: 10.3390/mi11030246.
  7. M. J. Männel, N. Hauck, J. Thiele*: „Solvent-resistant microfluidic devices made from PFHDA resins by micro-stereolithography“, Proc. SPIE 11235, Microfluidics, BioMEMS, and Medical Microsystems XVIII 2020, 112350H. DOI: 10.1117/12.2546060.
  8. T. Heida, T. Köhler, A. Kaufmann, M. J. Männel, J. Thiele*: „Cell-free protein synthesis in bifunctional hyaluronan hydrogel particles: A strategy for in situ immobilization and purification of His-tagged proteins“, ChemSystemsChem 2020, 2(3), e1900058. DOI: 10.1002/syst.201900058.
  9. D. Husman, P. B. Welzel, S. Vogler, L. J. Bray, N. Träber, J. Friedrichs, V. Körber, M. V. Tsurkan, U. Freudenberg, J. Thiele, C. Werner*: „A multi-parametric, microgel-in-gel platform to engineer cellular mesoenvironments“, Biomater. Sci. 2020, 8, 101. DOI: 10.1039/C9BM01009B.


  1. J. W. Neubauer, N. Hauck, M. J. Männel, A. Fery*, J. Thiele*: „Mechanoresponsive hydrogel particles as a platform for 3D-force sensing“, ACS Appl. Mater. Interfaces 2019, 11(29), 26307. DOI: 10.1021/acsami.9b04312.
  2. S. Rother*, V. Krönert, N. Hauck, A. Berg, S. Moeller, M. Schnabelrauch, J. Thiele, D. Scharnweber, V. Hintze: „Hyaluronan/collagen hydrogel matrices containing high-sulfated hyaluronan microgels for regulating transforming growth factor-β1“, J. Mater. Sci. – Mater. Med. 2019, 30, 65. DOI: 10.1007/s10856-019-6267-1.
  3. M. J. Männel, N. Weigel, J. Thiele*: „Multifunctional microfluidic devices from tailored photopolymer formulations“, Proc. SPIE 10875, Microfluidics, BioMEMS, and Medical Microsystems XVII 2019, 1087507. DOI: 10.1117/12.2514798.
  4. M. J. Männel, L. Selzer, R. Bernhardt, J. Thiele*: „Optimizing process parameters in commercial micro–stereolithography for forming emulsions and polymer microparticles in non–planar microfluidic devices“, Adv. Mater. Technol. 2019, 4, 1800408. DOI: 10.1002/admt.201800408.


  1. N. Hauck, N. Seixas, S. P. Centeno, R. Schlüßler, G. Cojoc, P. Müller, J. Guck, D. Wöll, L. A. Wessjohann*, J. Thiele*: „Droplet-assisted microfluidic fabrication and characterization of multifunctional polysaccharide microgels formed by multicomponent reactions“, Polymers 2018, 10, 1055. DOI: 10.3390/polym10101055.
  2. S. Girardo, N. Traeber, K. Wagner, G. Cojoc, C. Herold, R. Goswami, R. Schluessler, S. Abuhattum, A. Taubenberger, F. Reichel, D. Mokbel, M. Herbig, M. Schuermann, P. Mueller, T. Heida, A. Jacobi, J. Thiele, C. Werner, J. Guck*: “Standardized microgel beads as elastic cell mechanical probes”, J. Mater. Chem. B 2018, 6, 6245. DOI: 10.1039/C8TB01421C.


  1. J. Thiele*: “Polymer material design by microfluidics inspired by cell biology and cell-free biotechnology”, Macromol. Chem. Phys. 2017, 218, 1600429. DOI: 10.1002/macp.201600429.
  2. T. Heida, J. W. Neubauer, M. Seuss, N. Hauck, J. Thiele*, A. Fery*: “Mechanically defined microgels by droplet microfluidics”, Macromol. Chem. Phys. 2017, 218, 1600418. DOI: 10.1002/macp.201600418.


  1. M. M. K. Hansen, S. Paffenholz, D. Foschepoth, H. A. Heus, J. Thiele*, W. T. S. Huck*: „Cell-like nanostructured environments alter diffusion and reaction kinetics in cell-free gene expression”, ChemBioChem 2016, 17, 228. DOI: 10/1002/cbic.201500560.
  2. O. Wagner, J. Thiele, M. Weinhart, L. Mazutis, D. A. Weitz, W. T. S. Huck, R. Haag*: „Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants”, Lab Chip 2016, 16, 65. DOI: 10.1039/C5LC00823A.


  1. A. Gang, N. Haustein, L. Baraban, W. Weber, T. Mikolajick, J. Thiele*, G. Cuniberti*: „Microfluidic alignment and trapping of 1D nanostructures – a simple fabrication route for single-nanowire field effect transistors“, RSC Adv. 2015, 5, 94702. DOI: 10.1039/C5RA20414C.


  1. Y. Ma, M. P. Neubauer, J. Thiele, A. Fery, W. T. S. Huck*: “Artificial microniches for probing mesenchymal stem cell fate in 3D”, Biomater. Sci. 2014, 2, 1661. DOI: 10.1039/C4BM00104D.
  2. J. Thiele*, Y. Ma, D. Foschepoth, M. M. K. Hansen, C. Steffen, H. A. Heus, W. T. S. Huck*: “DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation”, Lab Chip 2014, 14, 2651. (2014 Lab Chip Emerging Investigators). DOI: 10.1039/c3lc51427g.
  3. V. Chokkalingam, Y .Ma, J. Thiele, W. Schalk, J. Tel, W. T. S. Huck*: “An electro-coalescence chip for effective emulsion breaking in droplet microfluidics”, Lab Chip 2014, 14, 2398. DOI: 10.1039/c4lc00365a.
  4. J. Thiele*, Y. Ma, S. M. C. Bruekers, S. Ma, W. T. S. Huck*: “Designer hydrogels for cell cultures: A materials selection guide”, Adv. Mater. 2014, 26, 125. DOI: 10.1002/adma.201302958.
  5. J. Thiele*, V. Chokkalingam, S. Ma, D. A. Wilson, W. T. S. Huck*: „Vesicle budding from polymersomes templated by microfluidically prepared double emulsions“, Mater. Horiz. 2014, 1, 96. DOI: 10.1039/C3MH00043E.
  6. Y. Ma, J. Thiele, L. Abdelmohsen, J. Xu, W. T. S. Huck*: “Biocompatible macro-initiators controlling radical retention in microfluidic on-chip photo-polymerization of water-in-oil emulsions”, Chem. Commun. 2014, 4, 112. DOI: 10.1039/C3CC46733C.


  1. V. Chokkalingam, J. Tel, F. Wimmers, X. Liu, S. Semenov, J. Thiele, C. G. Figdor, W. T. S. Huck*: „Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics“, Lab Chip 2013, 13, 4740. DOI: 10.1039/c3lc50945a.
  2. M. Trebbin, D. Steinhauser, J. Perlich, A. Buffet, S. V. Roth, W. Zimmermann, J. Thiele*, S. Förster*: “Anisotropic particles align perpendicular to the flow-direction in narrow microchannels”, Proc. Natl. Acad. Sci. U.S.A. 2013, 110, 6706. DOI: .


  1. W. J. Duncanson, T. Lin, A. R. Abate, R. K. Shah, D. A. Weitz*, J. Thiele: “Microfluidic synthesis of advanced microparticles for encapsulation and controlled release”, Lab Chip 2012, 12, 2135-2145; Lab Chip 2012, 12, 5279.
  2. S. Ma, J. Thiele, X. Liu, Y. Bai, C. Abell, W. T. S. Huck*: “Fabrication of microgel particles with complex shape via selective polymerization of aqueous two-phase systems”, Small 2012, 8, 2356. DOI: 10.1002/smll.201102715.
  3. T. Liu, S. Seiffert, J. Thiele, A. R. Abate, D. A. Weitz, W. Richtering*: “Non-coalescence of oppositely charged droplets in pH-sensitive emulsions”, Proc. Natl. Acad. Sci. U.S.A. 2012, 109, 384. DOI: .


  1. A. R. Abate, A. Rotem, J. Thiele, D. A. Weitz*: “Efficient encapsulation with plug-triggered drop formation”, Phys. Rev. E 2011, 84, 031502. DOI: 10.1103/PhysRevE.84.031502.
  2. J. Thiele*, S. Seiffert: “Double emulsions with controlled morphology by microgel scaffolding”, Lab Chip 2011, 11, 3188. DOI: 10.1039/c1lc20242a.
  3. J. Thiele*, M. Windbergs, A. R. Abate, M. Trebbin, H. C. Shum, S. Förster, D. A. Weitz*: “Early development drug formulation on a chip: “Fabrication of nanoparticles using a microfluidic spray dryer”, Lab Chip 2011, 11, 2362. DOI: 10.1039/c1lc20298g. (Featured in Chemistry World)
  4. A. R. Abate, J. Thiele, D. A. Weitz*: “One-step formation of multiple emulsions in microfluidics”, Lab Chip 2011, 11, 253. DOI: 10.1039/c0lc00236d.


  1. J. Thiele, A. R. Abate, H. C. Shum, S. Bachtler, S. Förster, D. A. Weitz*: “Fabrication of Polymersomes using Double-Emulsion Templates in Glass-Coated Stamped Microfluidic Devices”, Small 2010, 6, 1723. DOI: 10.1002/smll.201000798. (Featured in Materials View)
  2. S. Seiffert, J. Thiele, A. R. Abate, D. A. Weitz*: „Smart microgel capsules from macromolecular precursors“, J. Am. Chem. Soc. 2010, 132, 6606. DOI: 10.1021/ja102156h.
  3. A. R. Abate, J. Thiele, M. Weinhart, D. A. Weitz*: „Patterning microfluidic device wettability using flow confinement“, Lab Chip 2010, 10, 1774. DOI: 10.1039/C004124F.
  4. J. Thiele, D. Steinhauser, T. Pfohl, S. Förster: „Preparation of monodisperse block copolymer vesicles via flow focusing in microfluidics“, Langmuir 2010, 26, 6860. DOI: 10.1021/la904163v.
  5. H. C. Shum*, A. R. Abate, D. Lee, A. Studart, B. Wang, C. Chen, J. Thiele, R. K. Shah, A. Krummel, D. A. Weitz*: „Droplet Microfluidics for Fabrication of Non-Spherical Particles“, Macromol. Rapid Commun. 2010, 31, 108. DOI: 10.1002/marc.200900590.


  1. U. E. A. Fittschen*, F. Meirer, C. Streli, P. Wobrauschek, J. Thiele, G. Falkenberg, G. Pepponi: „Characterization of atmospheric aerosols using Synchroton radiation total reflection X-ray fluorescence and Fe K-edge total reflection X-ray fluorescence-X-ray absorption near-edge structure“, Spectrochim. Acta Part B 2008, 63, 1489. DOI: 10.1016/j.sab.2008.10.016.
  2. U. E. A. Fittschen*, N. H. Bings, S. Hauschild, S. Förster, A. F. Kiera, E. Karavani, A. Frömsdorf, J. Thiele, G. Falkenberg: „Characteristics of Picoliter Droplet Dried Residues as Standards for Direct Analysis Techniques“, Anal. Chem. 2008, 80, 1967. DOI: 10.1021/ac702005x.


  1. J. Thiele, M. J. Männel, N. Weigel: „Verfahren zur Herstellung von material- und/oder funktionalstrukturierten Bauteilen“, 2021, Patentanmeldung 10 2021 100 813.1.
  2. D. A. Weitz, J. Thiele, A. R. Abate: „Multiple Emulsions Created Using Jetting and Other Techniques“, 2020, Patent US 10874997 B2.
  3. A. R. Abate, J. Thiele, D. A. Weitz, C. Holtze, M. Windbergs: „Spray drying techniques“, 2016, Patent Pub. No. US 2016/0023126 A1.
  4. J. Thiele, G. Cuniberti: „Mikrofluidische Flusszelle, Verfahren zur Synthese von Biomolekülen in einer mikrofluidischen Flusszelle und Verfahren zur Herstellung einer mikrofluidischen Flusszelle“, 2015, Patent DE 102015113887 B3.


  1. J. Thiele, S. Seiffert: „Microfluidics: Theory and Practice for Beginners“, in Graduate Textbook Series, DeGruyter 2019, 295 pp. Link to DeGruyter Textbook Microfluidics
  2. N. Hauck, C. Graf, J. Thiele: „Zellfreie Biotechnologie – Polymermikrogele als experimentelle Plattform“, GIT Labor-Fachzeitschrift 2018, 08/18, 23.
  3. S. Seiffert, B. Esser, J. Thiele, R. Staff, C. Holtze, S. Koltzenburg, G. Oenbrink, N. Nestle: „Trendberichte Makromolekulare Chemie 2014“, Nachrichten aus der Chemie 2015, 3, 336.
  4. H. C. Shum, J. Thiele, S.-H. Kim: “Microfluidic fabrication of vesicles”, Advances in Transport Phenomena 2011, Volume 3, L. Wang (Ed.), Springer, 2014, 1.
  5. J. Thiele, S. Förster: „Unexpected flow behavior of anisotropic particles“, DESY Photon Science – Research Highlights 2013, 26.
  6. Six HASYLAB Annual Reports (German Electron Synchrotron DESY)

Google Scholar: h-Index 23, i10-index 30, 2.600 citations (23.01.2021)

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