Research group ThieleLab

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  1. J. Thiele*: „The challenge of cytocompatibility in high-resolution additive manufacturing“, in preparation.
  2. Y. Li*, Y. Du, B. Zhao, H. P. Patel, R. Boldt, Y. Dong, G. Auernhammer, A. Fery, J. Thiele*: „Titanium dioxide nanoparticles embedded in dipeptide hydrogels using microfluidics for photodegradation“, in preparation.
  3. A. Kaufmann, C. Graf, S. Manoilenko, M. Milic, L. Schäfer, M. Männel, N. Hauck, T. Heida, D. Hahn, M. Dippe, J. Thiele*: „Microbioreactors for polyketide synthesis based on enzyme-functionalized microgel cascades“, in preparation.
  4. A. Kaufmann*, M. Vigogne, T. A. Neuendorf, H. López-Menéndez, G. Rivas Caballero, J. Thiele: „Studying SBScons-SlmA interaction involved in bacterial cell division using polymer microgels as nucleoid mimics“, in preparation.


  1. M. M. Ghelardini, M. Geisler, N. Weigel, J. P. Hankwitz, N. Hauck, J. Schubert, A. Fery, J. B. Tracy*, J. Thiele*: „3D-Printed Hydrogels as Photothermal Actuators“, submitted.
  2. N. Weigel, E. Grigoryev, N. Fertala, J. Thiele*: „Fabrication of thermoresponsive and multimaterial hydrogel sheets by spatially controlled aspiration and interconnection of microgel building blocks“, Adv. Mater. Technol. 2023, accepted.
  3. N. Weigel, Y. Li, J. Thiele*, A. Fery: „From microfluidics to hierarchical hydrogel materials“, COCIS 2023, revised.


  1. N. Hauck, G. Cojoc, T. Beck, R. Schlüßler, S. Ahmed, S. Abuhattum, I. Raguzin, M. Mayer, J. Schubert, P. Müller, J. Guck, J. Thiele*: „PNIPAAm microgels with defined network architecture for temperature sensing in optical stretchers“, Mater. Adv. 2022, 3, 6179. DOI: 10.1039/d2ma00296e.
  2. D. Rettke, C. Danneberg, T. A. Neuendorf, S. Kühn, J. Friedrichs, N. Hauck, C. Werner, J. Thiele, T. Pompe*: „Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors“, J. Mater. Chem. B 2022, 10, 1663. DOI: 10.1039/D1TB02798K.
  3. T. A. Neuendorf, N. Weigel, M. Vigogne, J. Thiele*: „Additive soft matter design by UV-induced polymer hydrogel inter-crosslinking“, Gels 2022, 8(2), 117. DOI: 10.3390/gels8020117.
  4. E. Stengelin, J. Thiele, S. Seiffert*: „Multiparametric material functionality of microtissue-based in-vitro models as alternatives to animal testing“, Adv. Sci. 2022, 9(10), 2105319. DOI: 10.1002/advs.202105319.


  1. N. Hauck, T. A. Neuendorf, M. J. Männel, L. Vogel, P. Liu, E. Stündel, Y. Zhang, J. Thiele*: „Processing of fast-gelling hydrogel precursors in microfluidics by electrocoalescence of reactive species“, Soft Matter 2021, 17, 10312. DOI: 10.1039/d1sm01176f.
  2. M. J. Männel, N. Weigel, N. Hauck, T. Heida, J. Thiele*: „Combining Hydrophilic and Hydrophobic Materials in 3D printing for Fabricating Microfluidic Devices with Spatial Wettability“, Adv. Mater. Technol. 2021, 6(9), 2100094. DOI: 10.1002/admt.202100094.
  3. N. Weigel, M. J. Männel, J. Thiele*: „Flexible materials for high-resolution 3D printing of microfluidic devices with integrated droplet size regulation“, ACS Appl. Mater. Interfaces 2021, 13, 31086. DOI: 10.1021/acsami.1c05547.
  4. M. J. Männel, E. Baysak, J. Thiele*: „Fabrication of microfluidic devices for emulsion formation by micro-stereolithography“, Molecules 2021, 26(9), 2817. DOI: 10.3390/molecules26092817.
  5. Y. Li*, M. J. Männel, N. Hauck, H. P. Patel, G. K. Auernhammer, S. Chae, A. Fery, J. Li*, J. Thiele*: „Embedment of quantum dots and biomolecules in an in-situ formed dipeptide hydrogel using microfluidics“, Angew. Chem. Int. Ed. 2021, 60(12), 6724. DOI: 10.1002/ange.202015340.
  6. D. Eigel, R. Schuster, M. J. Männel, J. Thiele, M. Panasiuk, L. Andrae, C. Varracchio, A. Brancale, P. B. Welzel, W. Huttner, C. Werner, B. Newland*, K. R. Long*: „Sulfonated cryogel scaffolds for focal delivery in ex-vivo brain tissue cultures“, Biomaterials 2021, 271, 120712. DOI: 10.1016/j.biomaterials.2021.120712.
  7. N. Weigel, J. Thiele*: „Photopolymer formulations for μSL printing of hydrogel microstructures as swellable functional elements“, Proc. SPIE 11637, Microfluidics, BioMEMS, and Medical Microsystems XIX 2021, 116370A. DOI: 10.1117/12.2576485.


  1. T. Köhler, T. Heida, S. Hoefgen, N. Weigel, V. Valiante, J. Thiele*: „Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion“, RSC Adv. 2020, 10, 40588. DOI: 10.1039/d0ra06702d.
  2. C. Huster, D. Rekhade, A. Hausch, S. Ahmed, N. Hauck, J. Thiele, J. Guck, K. Kroy, G. Cojoc*: „Stretching and Heating Cells with Light Nonlinear Photothermal Cell Rheology“, New J. Phys. 2020, 22, 085003. DOI: 10.1088/1367-2630/aba14b.
  3. T. Heida, O. Otto, D. Biedenweg, N. Hauck, J. Thiele*: „Microfluidic fabrication of click chemistry-mediated hyaluronic acid microgels: A bottom-up material guide to tailor a microgel’s physicochemical and mechanical properties“, Polymers 2020, 12(8), 1760. DOI: 10.3390/polym12081760.
  4. J. E. Kufs, S. Hoefgen, J. Rautschek, A. U. Bissell, C. Graf, J. Fiedler, D. Braga, L. Regestein, M. A. Rosenbaum, J. Thiele, V. Valiante*: „Rational design of flavonoid production routes using combinatorial and precursor-directed biosynthesis“, ACS Synth. Biol. 2020, 9, 1823. DOI: 10.1021/acssynbio.0c00172.
  5. T. Köhler, P. A. Patsis, D. Hahn, A. Ruland, M. Müller, J. Thiele*: „DNAzymes as catalysts for L-tyrosine and amyloid beta oxidation“, ACS Omega 2020, 5(13), 7059. DOI: 10.1021/acsomega.9b02645.
  6. M. J. Männel, C. Fischer, J. Thiele*: „A Non-Toxic Resin for micro-stereolithography for Cell Cultures of HUVECs“, Micromachines 2020, 11(3), 246. DOI: 10.3390/mi11030246.
  7. M. J. Männel, N. Hauck, J. Thiele*: „Solvent-resistant microfluidic devices made from PFHDA resins by micro-stereolithography“, Proc. SPIE 11235, Microfluidics, BioMEMS, and Medical Microsystems XVIII 2020, 112350H. DOI: 10.1117/12.2546060.
  8. T. Heida, T. Köhler, A. Kaufmann, M. J. Männel, J. Thiele*: „Cell-free protein synthesis in bifunctional hyaluronan hydrogel particles: A strategy for in situ immobilization and purification of His-tagged proteins“, ChemSystemsChem 2020, 2(3), e1900058. DOI: 10.1002/syst.201900058.
  9. D. Husman, P. B. Welzel, S. Vogler, L. J. Bray, N. Träber, J. Friedrichs, V. Körber, M. V. Tsurkan, U. Freudenberg, J. Thiele, C. Werner*: „A multi-parametric, microgel-in-gel platform to engineer cellular mesoenvironments“, Biomater. Sci. 2020, 8, 101. DOI: 10.1039/C9BM01009B.


  1. J. W. Neubauer, N. Hauck, M. J. Männel, A. Fery*, J. Thiele*: „Mechanoresponsive hydrogel particles as a platform for 3D-force sensing“, ACS Appl. Mater. Interfaces 2019, 11(29), 26307. DOI: 10.1021/acsami.9b04312.
  2. S. Rother*, V. Krönert, N. Hauck, A. Berg, S. Moeller, M. Schnabelrauch, J. Thiele, D. Scharnweber, V. Hintze: „Hyaluronan/collagen hydrogel matrices containing high-sulfated hyaluronan microgels for regulating transforming growth factor-β1“, J. Mater. Sci. – Mater. Med. 2019, 30, 65. DOI: 10.1007/s10856-019-6267-1.
  3. M. J. Männel, N. Weigel, J. Thiele*: „Multifunctional microfluidic devices from tailored photopolymer formulations“, Proc. SPIE 10875, Microfluidics, BioMEMS, and Medical Microsystems XVII 2019, 1087507. DOI: 10.1117/12.2514798.
  4. M. J. Männel, L. Selzer, R. Bernhardt, J. Thiele*: „Optimizing process parameters in commercial micro–stereolithography for forming emulsions and polymer microparticles in non–planar microfluidic devices“, Adv. Mater. Technol. 2019, 4, 1800408. DOI: 10.1002/admt.201800408.


  1. N. Hauck, N. Seixas, S. P. Centeno, R. Schlüßler, G. Cojoc, P. Müller, J. Guck, D. Wöll, L. A. Wessjohann*, J. Thiele*: „Droplet-assisted microfluidic fabrication and characterization of multifunctional polysaccharide microgels formed by multicomponent reactions“, Polymers 2018, 10, 1055. DOI: 10.3390/polym10101055.
  2. S. Girardo, N. Traeber, K. Wagner, G. Cojoc, C. Herold, R. Goswami, R. Schluessler, S. Abuhattum, A. Taubenberger, F. Reichel, D. Mokbel, M. Herbig, M. Schuermann, P. Mueller, T. Heida, A. Jacobi, J. Thiele, C. Werner, J. Guck*: “Standardized microgel beads as elastic cell mechanical probes”, J. Mater. Chem. B 2018, 6, 6245. DOI: 10.1039/C8TB01421C.


  1. J. Thiele*: “Polymer material design by microfluidics inspired by cell biology and cell-free biotechnology”, Macromol. Chem. Phys. 2017, 218, 1600429. DOI: 10.1002/macp.201600429.
  2. T. Heida, J. W. Neubauer, M. Seuss, N. Hauck, J. Thiele*, A. Fery*: “Mechanically defined microgels by droplet microfluidics”, Macromol. Chem. Phys. 2017, 218, 1600418. DOI: 10.1002/macp.201600418.


  1. M. M. K. Hansen, S. Paffenholz, D. Foschepoth, H. A. Heus, J. Thiele*, W. T. S. Huck*: „Cell-like nanostructured environments alter diffusion and reaction kinetics in cell-free gene expression”, ChemBioChem 2016, 17, 228. DOI: 10/1002/cbic.201500560.
  2. O. Wagner, J. Thiele, M. Weinhart, L. Mazutis, D. A. Weitz, W. T. S. Huck, R. Haag*: „Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants”, Lab Chip 2016, 16, 65. DOI: 10.1039/C5LC00823A.


  1. A. Gang, N. Haustein, L. Baraban, W. Weber, T. Mikolajick, J. Thiele*, G. Cuniberti*: „Microfluidic alignment and trapping of 1D nanostructures – a simple fabrication route for single-nanowire field effect transistors“, RSC Adv. 2015, 5, 94702. DOI: 10.1039/C5RA20414C.


  1. Y. Ma, M. P. Neubauer, J. Thiele, A. Fery, W. T. S. Huck*: “Artificial microniches for probing mesenchymal stem cell fate in 3D”, Biomater. Sci. 2014, 2, 1661. DOI: 10.1039/C4BM00104D.
  2. J. Thiele*, Y. Ma, D. Foschepoth, M. M. K. Hansen, C. Steffen, H. A. Heus, W. T. S. Huck*: “DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation”, Lab Chip 2014, 14, 2651. (2014 Lab Chip Emerging Investigators). DOI: 10.1039/c3lc51427g.
  3. V. Chokkalingam, Y .Ma, J. Thiele, W. Schalk, J. Tel, W. T. S. Huck*: “An electro-coalescence chip for effective emulsion breaking in droplet microfluidics”, Lab Chip 2014, 14, 2398. DOI: 10.1039/c4lc00365a.
  4. J. Thiele*, Y. Ma, S. M. C. Bruekers, S. Ma, W. T. S. Huck*: “Designer hydrogels for cell cultures: A materials selection guide”, Adv. Mater. 2014, 26, 125. DOI: 10.1002/adma.201302958.
  5. J. Thiele*, V. Chokkalingam, S. Ma, D. A. Wilson, W. T. S. Huck*: „Vesicle budding from polymersomes templated by microfluidically prepared double emulsions“, Mater. Horiz. 2014, 1, 96. DOI: 10.1039/C3MH00043E.
  6. Y. Ma, J. Thiele, L. Abdelmohsen, J. Xu, W. T. S. Huck*: “Biocompatible macro-initiators controlling radical retention in microfluidic on-chip photo-polymerization of water-in-oil emulsions”, Chem. Commun. 2014, 4, 112. DOI: 10.1039/C3CC46733C.


  1. V. Chokkalingam, J. Tel, F. Wimmers, X. Liu, S. Semenov, J. Thiele, C. G. Figdor, W. T. S. Huck*: „Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics“, Lab Chip 2013, 13, 4740. DOI: 10.1039/c3lc50945a.
  2. M. Trebbin, D. Steinhauser, J. Perlich, A. Buffet, S. V. Roth, W. Zimmermann, J. Thiele*, S. Förster*: “Anisotropic particles align perpendicular to the flow-direction in narrow microchannels”, Proc. Natl. Acad. Sci. U.S.A. 2013, 110, 6706. DOI: .


  1. W. J. Duncanson, T. Lin, A. R. Abate, R. K. Shah, D. A. Weitz*, J. Thiele: “Microfluidic synthesis of advanced microparticles for encapsulation and controlled release”, Lab Chip 2012, 12, 2135-2145; Lab Chip 2012, 12, 5279.
  2. S. Ma, J. Thiele, X. Liu, Y. Bai, C. Abell, W. T. S. Huck*: “Fabrication of microgel particles with complex shape via selective polymerization of aqueous two-phase systems”, Small 2012, 8, 2356. DOI: 10.1002/smll.201102715.
  3. T. Liu, S. Seiffert, J. Thiele, A. R. Abate, D. A. Weitz, W. Richtering*: “Non-coalescence of oppositely charged droplets in pH-sensitive emulsions”, Proc. Natl. Acad. Sci. U.S.A. 2012, 109, 384. DOI: .


  1. A. R. Abate, A. Rotem, J. Thiele, D. A. Weitz*: “Efficient encapsulation with plug-triggered drop formation”, Phys. Rev. E 2011, 84, 031502. DOI: 10.1103/PhysRevE.84.031502.
  2. J. Thiele*, S. Seiffert: “Double emulsions with controlled morphology by microgel scaffolding”, Lab Chip 2011, 11, 3188. DOI: 10.1039/c1lc20242a.
  3. J. Thiele*, M. Windbergs, A. R. Abate, M. Trebbin, H. C. Shum, S. Förster, D. A. Weitz*: “Early development drug formulation on a chip: “Fabrication of nanoparticles using a microfluidic spray dryer”, Lab Chip 2011, 11, 2362. DOI: 10.1039/c1lc20298g. (Featured in Chemistry World)
  4. A. R. Abate, J. Thiele, D. A. Weitz*: “One-step formation of multiple emulsions in microfluidics”, Lab Chip 2011, 11, 253. DOI: 10.1039/c0lc00236d.


  1. J. Thiele, A. R. Abate, H. C. Shum, S. Bachtler, S. Förster, D. A. Weitz*: “Fabrication of Polymersomes using Double-Emulsion Templates in Glass-Coated Stamped Microfluidic Devices”, Small 2010, 6, 1723. DOI: 10.1002/smll.201000798. (Featured in Materials View)
  2. S. Seiffert, J. Thiele, A. R. Abate, D. A. Weitz*: „Smart microgel capsules from macromolecular precursors“, J. Am. Chem. Soc. 2010, 132, 6606. DOI: 10.1021/ja102156h.
  3. A. R. Abate, J. Thiele, M. Weinhart, D. A. Weitz*: „Patterning microfluidic device wettability using flow confinement“, Lab Chip 2010, 10, 1774. DOI: 10.1039/C004124F.
  4. J. Thiele, D. Steinhauser, T. Pfohl, S. Förster: „Preparation of monodisperse block copolymer vesicles via flow focusing in microfluidics“, Langmuir 2010, 26, 6860. DOI: 10.1021/la904163v.
  5. H. C. Shum*, A. R. Abate, D. Lee, A. Studart, B. Wang, C. Chen, J. Thiele, R. K. Shah, A. Krummel, D. A. Weitz*: „Droplet Microfluidics for Fabrication of Non-Spherical Particles“, Macromol. Rapid Commun. 2010, 31, 108. DOI: 10.1002/marc.200900590.


  1. U. E. A. Fittschen*, F. Meirer, C. Streli, P. Wobrauschek, J. Thiele, G. Falkenberg, G. Pepponi: „Characterization of atmospheric aerosols using Synchroton radiation total reflection X-ray fluorescence and Fe K-edge total reflection X-ray fluorescence-X-ray absorption near-edge structure“, Spectrochim. Acta Part B 2008, 63, 1489. DOI: 10.1016/j.sab.2008.10.016.
  2. U. E. A. Fittschen*, N. H. Bings, S. Hauschild, S. Förster, A. F. Kiera, E. Karavani, A. Frömsdorf, J. Thiele, G. Falkenberg: „Characteristics of Picoliter Droplet Dried Residues as Standards for Direct Analysis Techniques“, Anal. Chem. 2008, 80, 1967. DOI: 10.1021/ac702005x.


  1. J. Thiele, M. J. Männel, N. Weigel: „Verfahren zur Herstellung von material- und/oder funktionalstrukturierten Bauteilen“, 2021, Patentanmeldung 10 2021 100 813.1.
  2. D. A. Weitz, J. Thiele, A. R. Abate: „Multiple Emulsions Created Using Jetting and Other Techniques“, 2020, Patent US 10874997 B2.
  3. A. R. Abate, J. Thiele, D. A. Weitz, C. Holtze, M. Windbergs: „Spray drying techniques“, 2016, Patent Pub. No. US 2016/0023126 A1.
  4. J. Thiele, G. Cuniberti: „Mikrofluidische Flusszelle, Verfahren zur Synthese von Biomolekülen in einer mikrofluidischen Flusszelle und Verfahren zur Herstellung einer mikrofluidischen Flusszelle“, 2015, Patent DE 102015113887 B3.


  1. J. Thiele, S. Seiffert: „Microfluidics: Theory and Practice for Beginners“, in Graduate Textbook Series, DeGruyter 2019, 295 pp. Link to DeGruyter Textbook Microfluidics
  2. N. Hauck, C. Graf, J. Thiele: „Zellfreie Biotechnologie – Polymermikrogele als experimentelle Plattform“, GIT Labor-Fachzeitschrift 2018, 08/18, 23.
  3. S. Seiffert, B. Esser, J. Thiele, R. Staff, C. Holtze, S. Koltzenburg, G. Oenbrink, N. Nestle: „Trendberichte Makromolekulare Chemie 2014“, Nachrichten aus der Chemie 2015, 3, 336.
  4. H. C. Shum, J. Thiele, S.-H. Kim: “Microfluidic fabrication of vesicles”, Advances in Transport Phenomena 2011, Volume 3, L. Wang (Ed.), Springer, 2014, 1.
  5. J. Thiele, S. Förster: „Unexpected flow behavior of anisotropic particles“, DESY Photon Science – Research Highlights 2013, 26.
  6. Six HASYLAB Annual Reports (German Electron Synchrotron DESY)

Google Scholar: h-Index 26, i10-index 40, 3.472 citations (30.01.2023)

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