New lysate prepared: Chi6 DNA makes all the difference
31.07.19: We have developed a new round of lysate for cell-free protein synthesis (CFPS) based on Escherichia coli. This time, we combined two things: sonication-based lysate preparation according to Levine et al. (DOI: 10.3791/58882) and utilization of Chi6 DNA to enhance expression of linear DNA by reducing nuclease activity of RecBCD complex (sequence of deGFP and background information according to DOI: 10.1186/1754-1611-4-8 and DOI: 10.1002/bit.26333). The figure shows the fluorescence of deGFP after expression by illumination with an UV-lamp (365 nm), which is 11-times higher if Chi6 DNA is supplemented to the lysate. This lysate is currently utilized in our group for CFPS in bifunctional microgels, to be published ASAP.
Poster displayed at MechanoChemBio 2019 (McGill, Montreal)
30.07.19: Thanks a lot to Kerstin Blank (https://www.mpikg.mpg.de/5357427/Director) and her coworker Isabell Tunn for organizing the transport of our poster to Montreal and putting it up for the session.
ThieleLab presented latest research at IUPAC Paris (05.07. – 12.07.)
23.07.19: Two colleagues from the ThieleLab, Max Männel (MSc) and Nicolas Hauck (MSc), presented their work:
Poster P275: „Droplet-assisted microfluidic fabrication and characterization of multifunctional polysaccharide microgels formed by Ugi four-component reaction“
Poster P277: „Microscopic polymer hydrogels loaded in microreactors to perform enzymatic cascades towards polyketide synthesis“
For more details, feel free to contact us.
Dr. Yue Li receives Humboldt fellowship
09.07.19: We are proud of our colleague Dr. Yue Li, who has been awarded a fellowship by the Humboldt Foundation (https://www.humboldt-foundation.de/web/humboldt-en.html) to start her work on a new project dealing with light harvesting in polymer gel networks in the ThieleLab and in the group of Prof. Dr. Andreas Fery (https://www.ipfdd.de/de/organisation/personal-homepages/prof-dr-andreas-fery/).
ThieleLab performing a Change of Course!
09.07.19: The Volkswagen Foundation is funding our vision towards functional polymers by combining material design and cell-free biocatalysis together with our collaboration partner Prof. Dr. Marion Ansorge-Schumacher at TU Dresden: https://tu-dresden.de/mn/biologie/mikro/mbt.
In conventional polymer design, function is either being introduced in the synthesis process or via post-functionalization employing standard organic synthesis routes. Although being only available in small numbers, the end-group of a polymer is predominantly the designated target for introducing functionality beyond intrinsic polymer properties, as in many cases, the non-functional polymer backbone is either too inert or cannot be targeted by functionalization reagents at pre-defined locations.
We will explore cell-free bio-catalysis based on pre-screened, tailored enzymes to modify hard-to-functionalize, low-reactive polymers with functional groups. Thus, the range of a polymer’s function and consequently of application will be significantly extended. The particular focus will not be on targeting the limited number of polymer end-groups, but on targeting the polymer’s backbone made of repetitive monomer units, which may allow for precisely tuning the number of functionalized positions. Most importantly, enzymes that have previously been utilized mainly to introduce functional groups to low-molecular weight substrates will be explored to target abiological/synthetic polymers with high molecular weight. This disruptive approach is envisioned to be transferable to improve performance, applicability and bioavailability of leading polymer systems. Tackling the challenge of an accelerating demand for highly specialized, multifunctional polymers by introducing biocatalytic functionalization to synthetic polymers is our change of course for polymer material design and cell-free biocatalysis, adding distinct new directions of research to both fields.
Georg Manecke Award for Julian Thiele
23.05.19: Dr. Julian Thiele is this year’s recipient of the Georg Manecke Award (Georg Manecke Foundation). He is recognized for linking macromolecular chemistry with applications in biotechnology as well as drug development. The prize will be awarded during the „Multivalency in Chemistry and Biology“ meeting at FU Berlin (09/30 – 10/02).
More information: https://www.gdch.de/gdch/stiftungen.html
Leibniz Research Award 2019 awarded to Wittko Francke during Leibniz Conference on Bioactive Compounds in Dresden
06.04.19: Prof. Dr. Dr. h.c. mult. Wittko Francke (Hamburg University) is this year’s recipient of the Leibniz Research Award 2019. Congratulations!! Further thanks go to our plenary speakers Prof. Dr. Ansorge-Schumacher, Prof. Dr. Wilhelm T. S. Huck and Dr. Julien Lefranc as well as to all other speakers and poster authors who contributed to the success of this year’s meeting. Special thanks go to Dr. Anika Kaufmann and Anna Rusznyak for their support!!
Special issue in POLYMERS now open for submission!
07.03.19: The special issue „Polymer Material Design by Microfluidics“ of the open-access journal POLYMERS (IF 2.935) is now open for submission. Guest edited by Julian Thiele and Sebastian Seiffert, the focus will be on most recent developments in the application of microflow cells fabricated from PDMS, glass or via additive manufacturing/3D printing to pave the way towards the development of polymer materials with physicochemical and mechanical properties tailored from the nano- to microscale that exhibit novel architecture and functions.
For more information: MDPI Polymers Special Issue „Polymer Material Design by Microfluidics“, or contact the guest editor: thiele[at]ipfdd.de
ThieleLab and colleagues from IPF Dresden visiting UCI Havana and the Embassy of Cuba in Berlin, Germany
05.03.19: As part of our BMBF-funded project „3Dfor3M: Additive manufacturing for medicine, microsystems, and innovative materials“, colleagues from the ThieleLab and IPF Dresden visited Cuba and its embassy in Berlin to set up a research network for future H2020 project proposals.
More information (in Spanish): Delegation visiting Embassy of Cuba in Berlin and Delegation from IPF visiting UCI Havana as part of BMBF-funded research network 3Dfor3M
Leibniz Application Laboratory registered at DRESDEN Concept Technology Portal
27.02.19: You can now find more information on the Leibniz Application Laboratory „Additive manufacturing/3D-printing“, founded by the ThieleLab, here:
Poster session at „Makromolekulares Kolloquium Freiburg“
21.02.19: Nicolas Hauck has presented our work on multi-component reactions to microfluidically fabricate hydrogel particles in a library-like fashion (cf. Polymers 2018, 10, 1055). Thanks again to our collaboration partners Jochen Guck (MPI Erlangen), Dominik Wöll (RWTH Aachen) and Ludger A. Wessjohann (Leibniz IPB Halle) for making this a success!
OPEN FOR REGISTRATION: Leibniz Conference on Bioactive Compounds
11.02.19: As co-organizers (together with the Leibniz Research Alliance Bioactive Compounds and Biotechnology), we cordially invite you to the upcoming Leibniz Conference on Bioactive Compounds 2019! Follow our invitation and present your research in a poster or a short talk and get in touch with other scientists.
Great!ipid4all / DAAD grant obtained
09.01.19: Nicolas Hauck and Julian Thiele have received an award for travel allowance fron the TUD Graduate Academy to initiate collaborations on mechanoresponsive polymer gels with the WeitzLab at Harvard (https://weitzlab.seas.harvard.edu/).
Volkswagen Foundation supporting ThieleLab
29.11.18: The ThieleLab has successfully aquired funding by the VW Foundation under the „Experiment!“ program to explore a novel approach towards polymer multi-material printing based on particle-based photopolymer formulations in the next 1.5 years.
Update 17.04.19: Press release by Leibniz IPF Dresden can be found under https://idw-online.de/de/news714337. Glad to have received the first shipment from Lithoz (Vienna) to get started.
ThieleLab seeking out for new collaborations on additive manufacturing as part of the BMBF project „3Dfor3M“ (Horizon 2020 with partners from North- and Central America)
26.11.18: As part of the project „3Dfor3M – Additive manufacturing for Medicine, Microsystems and intelligent Materials“, representatives from the ThieleLab together with other colleagues from the Leibniz IPF Dresden are currently traveling to Boston (attending the MRS Fall Meeting and the 77th New England Complex Fluids Workshop), Bozeman (Montana State University) and Montreal (Concordia University) to set up new collaborations in preparation for upcoming H2020 calls.
Our collaborators from YONSEI UNIVERSITY visit IPF Dresden
04.07.18: Prof. Jong-Souk Yeo from the School of Integrated Technology at Yonsei University and two of his students have visited IPF Dresden. As part of this collaboration, Jihye Lee (PhD candidate, School of Integrated Technology) has started an 8-week internship in our group to work on neuromodulation and neurorecording in polymer hydrogels (in further collaboration with Dr. Ivan Minev, BIOTEC Dresden).
PhD position open (starting date: 1st September 2018) CLOSED
28.05.18: The ThieleLab aims at further broadening its research acitivties on 3D-printing by micro-stereolithography (µSL). Particularly, we are interested in the synthesis of multifunctional polymer materials, which will form the basis for formulations / resins in µSL. In close collaboration with other group members as well as external partners, these pre-polymers / macromers will be processed into defined materials with tailored optical properties, mechanics as well as sensitivity towards external triggers. For that, we currently invite candidates to submit their application to join our group as a PhD student with a strong background in polymer synthesis and macromolecular chemistry.
Please send your application to thiele“at“ipfdd.de.
Professor-Franz-Brandstetter-Award for master thesis from ThieleLab
18.04.18: Lisa Schäfer, former group member and now pursuing her PhD thesis at the Helmholtz Center for Environmental Research – UFZ (Environmental Engineering and Biotechnology) has been awarded the Prof.-Franz-Brandstetter-Award for her outstanding master thesis on polymer microgels als experimental platform for enzymatic conversion of Malonate to Malonyl-CoA, which is a key building block for polyketides (http://www.leibniz-research-cluster.de/de/).
More information (in German): https://www.ipfdd.de/de/oeffentlichkeitsarbeit/aktuelles/news-detailseite/newsitem/1235/
Publication preprint on biorxiv available
29.03.18: We have co-authored a joint manuscript from TU Dresden, BIOTEC, IPF Dresden and Zellmechanik Dresden on the first example of rationally designed microgel particles as standardized calibration samples for cell mechanics measurements. The pdf is available here: http://biorxiv.org/cgi/content/short/290569v1
Lab technician position open (starting date: ASAP) CLOSED
Laboranten/in mit 40 Wochenstunden ab sofort (Deutsche Stellenbeschreibung: THIELELAB_Labororatory technician)
29.01.18: The ThieleLab is looking for a promising lab technician, who wants to work in a strongly interdisciplinary research team (biologists, chemists, biotechnicians), and is open for new tasks (e.g. Computer-aided design). As a key member of the group, the position focuses on the design of microflow cells by photo- and soft lithography for other group members as well as collaboration partners. Other daily tasks include the lab management (consumables, hazardous substances registry) and introduction of new lab members to safety installations.
PhD position open (starting date: 1st April 2018) CLOSED
29.01.18: The ThieleLab aims at broadening their research activities in the area of additive manufacturing by (micro-)stereolithography. Particularly, we look for candidates, who are already experienced in using 3D printers (e.g. Asiga Pico2, Envisiontec P4 Mini), and want to apply their knowledge in a holistic approach to design novel photopolymers for 3D-printing, modify existing printing techniques, and ultimately design polymer materials unifying structure and function in one step. Please send you applications to thiele“at“ipfdd.de. More information: THIELELAB_PhD position_3D printing
ThieleLab part of the DFG Graduate School 1865
23.01.18: The ThieleLab is taking part in the second round of the Graduate School 1865 „Hydrogel-based microsystems“, funded by the DFG. The goal will be the design of microgels as voxel materials in additive manufacturing (particularly stereolithography). PhD position to be posted ASAP (start date: 01.04.18) ! More information on the graduate school an be found here: https://tu-dresden.de/ing/elektrotechnik/ife/graduiertenkolleg
Research group at Christmas Bowling
13.12.17: The Polymer micro(bio)reactors group improved their bowling skills 😉
Leibniz Application Lab online!
28.11.17: We are happy to announce the launch of the Leibniz Application Laboratory „Additive manufacturing / 3D printing“:
Special thanks to Stefan Schwurack for organization and web design.
New 3D printer in the ThieleLab!
17.11.17: After installation of an ASIGA Pico2 and an ENVISIONTEC Mini P4 ERM in 2016, we are proud to announce, that a LITHOZ CeraFab 7500 Mod has been added to the portfolio of 3D printers in our group. The microstereolithography printer with 10.4 µm resolution will be employed in our research „From resins to functional components“. For more information: thiele“at“ipfdd.de
Research project available for internship or master thesis!
04.10.17: „Synthesis and characterization of novel triblock compolymer surfactants based on poly(2-oxazoline)-block-poly(ethylene glycol)“. Please get in touch with us: heida“at“ipfdd.de or thiele“at“ipfdd.de.
Master theses defended!
29.09.17: Simon Baumgarte and Lisa Schäfer have obtained their master degree (BIOTEC, Molecular Bioengineering) after defending their thesis with great success. Congratulations to both of them 🙂
Research group on hiking trip 🙂
11.07.17: The Polymer micro(bio)reactors group explored the Dresdner Heide.
Julian Thiele announced as TU Dresden Young Investigator
10.05.17: Julian Thiele received his letter of appointment as Young Investigator at TU Dresden from Prof. Hans-Müller Steinhagen (rector of TUD). (Picture copyright Birgit Holthaus)